Isoform-specific and ubiquitination dependent recruitment of Tet1 to replicating heterochromatin modulates methylcytosine oxidation

A short isoform of the Tet1 enzyme (Tet1s) that oxidizes the DNA 5-methylcytosine mark has been found to be overexpressed in tumors. Here the authors show that Tet1s, but not full-length Tet1, changes localization throughout the cell cycle upon ubiquitination and Uhrf1 interaction and is targeted to heterochromatin during S-phase. This leads to spreading of 5-methylcytosine oxidation and loss of DNA methylation in heterochromatin.

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