Yeast Membrane Biology

Apl. Prof. Dr. Adam Bertl

Our lab is interested in structure, function and regulation of molecular machines that mediate transport of small molecules across biological membranes.

By combining the power of yeast genetics and the sensitivity of biophysical analysis, we are studying function of endogenous yeast membrane proteins (ion channels, cotransporter, ion pumps and water channels) in plasma membrane and tonoplast. For that we developed (adopted to yeast) and successfully applied methods – mainly patch-clamp – for recording electrical charge translocation on the level of individual transport proteins (ion channels), as well as on the macroscopic level, i.e. mediated by a whole population of transporters.

More recently, we established protocols in yeast for analysing transmembrane transport of uncharged substrates, such as water or gases (CO2, NH3). These methods use stopped-flow techniques in combination with light scattering/fluorescence spectroscopy and are intended to study (and understand) important steps in osmoregulation in yeast.

Besides our major interest in yeast membrane transport itself, we are using yeast as a heterologous expression system for functional identification and characterization of membrane transport proteins of plant and animal origin.

Jump to: 2019 | 2018 | 2017 | 2015 | 2013 | 2011 | 2010 | 2008 | 2007 | 2005 | 2004 | 2003 | 2002 | 1999 | 1998 | 1997 | 1996 | 1995 | 1994 | 1993 | 1992 | 1990 | 1989 | 1988 | 1987 | 1986 | 1985 | 1984
Number of items at this level (without sub-levels): 64.

2019

Degreif, Daniel and Cucu, Bayram and Budin, Itay and Thiel, Gerhard and Bertl, Adam (2019):
Lipid determinants of endocytosis and exocytosis in budding yeast.
In: Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864 (7), pp. 1005-1016. ISSN 1879-2618,
DOI: 10.1016/j.bbalip.2019.03.007,
[Article]

Bracharz, Felix and Karava, Marianna and Kabisch, Johannes (2019):
Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating.
In: PLOS ONE, 14 (7), PLOS, ISSN 1932-6203,
DOI: 10.1371/journal.pone.0219892,
[Article]

2018

Amini, Mahnaz and Wang, Hongmei and Belkacemi, Anouar and Jung, Martin and Bertl, Adam and Schlenstedt, Gabriel and Flockerzi, Veit and Beck, Andreas (2018):
Identification of Inhibitory Ca2+ Binding Sites in the Upper Vestibule of the Yeast Vacuolar TRP Channel.
In: iScience, 11, pp. 1-12. ISSN 2589-0042,
[Article]

Cucu, Bayram (2018):
Endocytose und Exocytose in Saccharomyces cerevisiae.
Darmstadt, Technische Universität,
[Ph.D. Thesis]

Degreif, Daniel (2018):
Metabolic engineering approaches reveal widespread physiological functions of membrane lipids for Saccharomyces cerevisiae.
Darmstadt, Technische Universität,
[Ph.D. Thesis]

Degreif, Daniel and Kremenovic, Milana and Geiger, Thomas and Bertl, Adam (2018):
Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.
In: Journal of Biological Methods, 5 (3), p. 98. ISSN 2326-9901,
DOI: 10.14440/jbm.2018.254,
[Article]

Uzcátegui, Néstor L. and Figarella, Katherine and Segnini, Andrea and Marsiccobetre, Sabrina and Lang, Florian and Beitz, Eric and Rodríguez-Acosta, Alexis and Bertl, Adam (2018):
Trypanosoma brucei aquaglyceroporins mediate the transport of metabolic end-products: Methylglyoxal, D-lactate, L-lactate and acetate.
In: Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860 (11), pp. 2252-2261. ISSN 00052736,
DOI: 10.1016/j.bbamem.2018.09.008,
[Article]

2017

Cucu, Bayram and Degreif, Daniel and Bertl, Adam and Thiel, Gerhard (2017):
Vesicle fusion and fission in plants and yeast.
In: Cell calcium, 67, pp. 40-45. ISSN 1532-1991,
[Article]

Degreif, Daniel and de Rond, Tristan and Bertl, Adam and Keasling, Jay D. and Budin, Itay (2017):
Lipid engineering reveals regulatory roles for membrane fluidity in yeast flocculation and oxygen-limited growth.
In: Metabolic engineering, 41, pp. 46-56. ISSN 1096-7184,
[Article]

2015

Carrillo, Lucia (2015):
Application of high-resolution membrane capacitance measurements in the study of exocytosis and endocytosis in Saccharomyces cerevisiae.
Darmstadt, Technische Universität,
[Ph.D. Thesis]

Carrillo, Lucia and Cucu, Bayram and Bandmann, Vera and Homann, Ulrike and Hertel, Brigitte and Hillmer, Stefan and Thiel, Gerhard and Bertl, Adam (2015):
High-resolution membrane capacitance measurements for studying endocytosis and exocytosis in yeast.
In: Traffic (Copenhagen, Denmark), 16 (7), pp. 760-772. ISSN 1398-9219,
[Article]

Cucu, Bayram and Carillo, Lucia and Degreif, Daniel and Kohl, Max and Thiel, Gerhard and Bertl, Adam (2015):
The role of Clc1p and Chc1p in endocytosis/exocytosis in yeast.
In: Yeast, 32, pp. S116. ISSN 0749-503X,
[Article]

Degreif, Daniel and Bertl, Adam and Keasling, Jay D. and Budin, Itay (2015):
A novel flocculation pathway mediated by ER membrane fluidity.
In: Yeast, 32, pp. S117. ISSN 0749-503X,
[Article]

Safiarian, Minou J. and Pertl-Obermeyer, Heidi and Lughofer, Peter and Hude, Rene and Bertl, Adam and Obermeyer, Gerhard (2015):
Lost in traffic? The K(+) channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves, and lily pollen.
In: Frontiers in plant science, 6, p. 47. ISSN 1664-462X,
[Article]

2013

Bertl, Adam and Carrillo, Lucia and Koehler, Tim and Bandmann, Vera and Ayaz, Muhammad and Homann, Ulrike (2013):
Real-time recording of individual endocytotic and exocytotic events in yeast using the patch-clamp techniques.
In: Yeast, 30, p. 114. ISSN 0749-503X,
[Article]

2011

Obermeyer, Gerhard and Bertl, Adam (2011):
Das Membranpotenzial : Biophysik im Experiment.
In: Biologie in unserer Zeit, 41 (3), pp. 206-211. [Article]

2010

Cosentino, C. and Fischer-Schliebs, E. and Bertl, A. and Thiel, Gerhard and Homann, Ulrike (2010):
Na+/H+ antiporters are differentially regulated in response to NaCl stress in leaves and roots of Mesembryanthemum crystallinum.
In: The New phytologist, 186 (3), pp. 669-680. ISSN 1469-8137,
[Article]

2008

Roller, A. and Natura, G. and Bihler, H. and Slayman, C. L. and Bertl, A. (2008):
Functional consequences of leucine and tyrosine mutations in the dual pore motifs of the yeast K(+) channel, Tok1p.
In: Pflügers Archiv : European journal of physiology, 456 (5), pp. 883-896. ISSN 0031-6768,
[Article]

2007

Alpi, A. and Amrhein, N. and Bertl, A. and Blatt, M. R. and Blumwald, E. and Cervone, F. and Dainty, J. and De Michelis, M. I. and Epstein, E. and Galston, A. W. and Goldsmith, M. H. M. and Hawes, C. and Hellwig, R. and Hetherington, A. and Hofte, H. and Juergens, G. and Leaver, C. J. and Moroni, A. and Murphy, A. and Oparka, K. and Perata, P. and Quader, H. and Rauscher, T. and Ritzenthaler, C. and Rivetta, A. and Robinson, D. G. and Sanders, D. and Scheres, B. and Schumacher, K. and Sentenac, H. and Slayman, C. L. and Soave, C. and Somerville, C. and Taiz, L. and Thiel, Gerhard and Wagner, R. (2007):
Plant neurobiology: no brain, no gain?
In: Trends in plant science, 12 (4), pp. 135-136. ISSN 1360-1385,
[Article]

Bertl, Adam and Kaldenhoff, Ralf (2007):
Function of a separate NH3-pore in Aquaporin TIP2;2 from wheat.
In: FEBS letters, 581 (28), pp. 5413-5417. ISSN 0014-5793,
[Article]

Gehwolf, R. and Weiss, R. and Gabler, M. and Hurst, A. C. and Bertl, A. and Thalhamer, J. and Obermeyer, G. (2007):
From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.
In: FEBS letters, 581 (3), pp. 448-52. ISSN 0014-5793,
[Article]

Kaldenhoff, Ralf and Bertl, Adam and Otto, Beate and Moshelion, M. and Uehlein, Norbert (2007):
Characterization of plant aquaporins.
In: Methods in enzymology, 428, pp. 505-531. ISSN 0076-6879,
[Article]

Latz, A. and Becker, D. and Hekman, M. and Müller, T. and Beyhl, D. and Marten, I. and Eing, C. and Fischer, A. and Dunkel, M. and Bertl, A. and Rapp, U. R. and Hedrich, R. (2007):
TPK1, a Ca(2+)-regulated Arabidopsis vacuole two-pore K(+) channel is activated by 14-3-3 proteins.
In: The Plant journal : for cell and molecular biology, 52 (3), pp. 449-459. ISSN 0960-7412,
[Article]

Uehlein, Norbert and Fileschi, K. and Eckert, M. and Bienert, G. P. and Bertl, Adam and Kaldenhoff, Ralf (2007):
Arbuscular mycorrhizal symbiosis and plant aquaporin expression.
In: Phytochemistry, 68 (1), pp. 122-129. ISSN 0031-9422,
[Article]

2005

Bihler, H. and Eing, C. and Hebeisen, S. and Roller, A. and Czempinski, K. and Bertl, A. (2005):
TPK1 is a vacuolar ion channel different from the slow-vacuolar cation channel.
In: Plant physiology, 139 (1), pp. 417-24. ISSN 0032-0889,
[Article]

Porée, F. and Wulfetange, K. and Naso, A. and Carpaneto, A. and Roller, A. and Natura, G. and Bertl, A. and Sentenac, H. and Thibaud, J. and Dreyer, I. (2005):
Plant K(in) and K(out) channels: approaching the trait of opposite rectification by analyzing more than 250 KAT1-SKOR chimeras.
In: Biochemical and biophysical research communications, 332 (2), pp. 465-73. ISSN 0006-291X,
[Article]

Roller, A. and Natura, G. and Bihler, H. and Slayman, C. L. and Eing, C. and Bertl, A. (2005):
In the yeast potassium channel, Tok1p, the external ring of aspartate residues modulates both gating and conductance.
In: Pflügers Archiv : European journal of physiology, 451 (2), pp. 362-370. ISSN 0031-6768,
[Article]

2004

Becker, D. and Geiger, D. and Dunkel, M. and Roller, A. and Bertl, A. and Latz, A. and Carpaneto, A. and Dietrich, P. and Roelfsema, M. R. G. and Voelker, C. and Schmidt, D. and Mueller-Roeber, B. and Czempinski, K. and Hedrich, R. (2004):
AtTPK4, an Arabidopsis tandem-pore K+ channel, poised to control the pollen membrane voltage in a pH- and Ca2+-dependent manner.
In: Proceedings of the National Academy of Sciences of the United States of America, 101 (44), pp. 15621-6. ISSN 0027-8424,
[Article]

Dreyer, I. and Porée, F. and Schneider, A. and Mittelstädt, J. and Bertl, A. and Sentenac, H. and Thibaud, J. and Mueller-Roeber, B. (2004):
Assembly of plant Shaker-like K(out) channels requires two distinct sites of the channel alpha-subunit.
In: Biophysical journal, 87 (2), pp. 858-872. ISSN 0006-3495,
[Article]

2003

Bertl, A. and Ramos, J. and Ludwig, J. and Lichtenberg-Fraté, H. and Reid, J. and Bihler, H. and Calero, F. and Martínez, P. and Ljungdahl, P. O. (2003):
Characterization of potassium transport in wild-type and isogenic yeast strains carrying all combinations of trk1, trk2 and tok1 null mutations.
In: Molecular microbiology, 47 (3), pp. 767-80. ISSN 0950-382X,
[Article]

Kettner, C. and Bertl, A. and Obermeyer, G. and Slayman, C. L. and Bihler, H. (2003):
Electrophysiological analysis of the yeast V-type proton pump: variable coupling ratio and proton shunt.
In: Biophysical journal, 85 (6), pp. 3730-3738. ISSN 0006-3495,
[Article]

Kettner, C. and Obermeyer, G. and Bertl, A. (2003):
Inhibition of the yeast V-type ATPase by cytosolic ADP.
In: FEBS letters, 535 (1-3), pp. 119-124. ISSN 0014-5793,
[Article]

2002

Bihler, H. and Slayman, C. L. and Bertl, A. (2002):
Low-affinity potassium uptake by Saccharomyces cerevisiae is mediated by NSC1, a calcium-blocked non-specific cation channel.
In: Biochimica et biophysica acta, 1558 (2), pp. 109-118. ISSN 0006-3002,
[Article]

1999

Bihler, H. and Gaber, R. F. and Slayman, C. L. and Bertl, A. (1999):
The presumed potassium carrier Trk2p in Saccharomyces cerevisiae determines an H+-dependent, K+-independent current.
In: FEBS letters, 447 (1), pp. 115-120. ISSN 0014-5793,
[Article]

Bihler, H. and Slayman, C. L. and Bertl, A. (1999):
Calcium-Inhibited Non-Selective Cation "Channels" (Nscl) Admit Large Inward Currents through the Yeast Plasma Membrane.
In: Folia Microbiologica, 44 (2), pp. 221-222. ISSN 1874-9356,
[Article]

1998

Bertl, A. and Bihler, H. and Kettner, C. and Slayman, C. L. (1998):
Electrophysiology in the eukaryotic model cell Saccharomyces cerevisiae.
In: Pflügers Archiv : European journal of physiology, 436 (6), pp. 999-1013. ISSN 0031-6768,
[Article]

Bertl, A. and Bihler, H. and Reid, J. D. and Kettner, C. and Slayman, C. L. (1998):
Physiological characterization of the yeast plasma membrane outward rectifying K+ channel, DUK1 (TOK1), in situ.
In: The Journal of membrane biology, 162 (1), pp. 67-80. ISSN 0022-2631,
[Article]

Bihler, H. and Slayman, C. L. and Bertl, A. (1998):
NSC1: a novel high-current inward rectifier for cations in the plasma membrane of Saccharomyces cerevisiae.
In: FEBS letters, 432 (1-2), pp. 59-64. ISSN 0014-5793,
[Article]

1997

Bertl, A. (1997):
Letter to the editor: The spTRK gene encodes a potassium-specific transport protein TKHp in Schizosaccharomyces pombe.
In: The Journal of membrane biology, 159 (1), pp. 95-7. ISSN 0022-2631,
[Article]

Bertl, A. and Reid, J. D. and Sentenac, H. and Slayman, C. L. (1997):
Functional comparison of plant inward rectifier channels expressed in yeast.
In: Journal of experimental botany, 48, pp. 405-413. ISSN 1460-2431,
[Article]

1996

Reid, J. D. and Lukas, W. and Shafaatian, R. and Bertl, A. and Scheurmann-Kettner, C. and Guy, H. R. and North, R. A. (1996):
The S. cerevisiae outwardly-rectifying potassium channel (DUK1) identifies a new family of channels with duplicated pore domains.
In: Receptors & channels, 4 (1), pp. 51-62. ISSN 1060-6823,
[Article]

Scheurmann-Kettner, C. and Gradmann, D. and Bertl, A. (1996):
Kinetic effects of extracellular calcium on the potassium outward rectifier in the plasma membrane of Saccharomyces cerevisiae.
In: Folia microbiologica, 41 (1), pp. 126-8. ISSN 0015-5632,
[Article]

1995

Bertl, A. and Anderson, J. A. and Slayman, C. L. and Gaber, R. F. (1995):
Use of Saccharomyces cerevisiae for patch-clamp analysis of heterologous membrane proteins: characterization of Kat1, an inward-rectifying K+ channel from Arabidopsis thaliana, and comparison with endogeneous yeast channels and carriers.
In: Proceedings of the National Academy of Sciences of the United States of America, 92 (7), pp. 2701-2705. ISSN 0027-8424,
[Article]

1994

Bertl, A. and Anderson, J. A. and Slayman, C. L. and Sentenac, H. and Gaber, R. F. (1994):
Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
In: Folia microbiologica, 39 (6), pp. 507-9. ISSN 0015-5632,
[Article]

Slayman, C. L. and Bertl, A. (1994):
Modes and means of channel behavior: an introduction.
In: Symposia of the Society for Experimental Biology, 48, pp. 77-83. ISSN 0081-1386,
[Article]

Slayman, C. L. and Bertl, A. and Blatt, M. R. Hirst, B. H. (ed.) Advanced Research Workshop on Molecular and Cellular Mechanisms of H + Transport <1993, York> (Corporate Creator) (1994):
Partial reaction chemistry and charge displacement by the fungal plasma-membrane H+-ATPase.
In: NATO ASI series : Ser. H, Cell biology, In: Molecular and cellular mechanisms of H+ transport : proceedings of the NATO Advanced Research Workshop on Molecular and Cellular Mechanisms of H+ Transport, held in York, UK, July 27 to August 1, 1993, pp. 237-244, Berlin, Springer Verlag, ISBN 3-540-58497-8,
[Book Section]

1993

Azzone, G. and Benz, R. and Bertl, A. and Colombini, M. and Crofts, A. and Dilley, R. and Dimroth, P. and Dutton, P. L. and Felle, H. and Harold, F. and Junge, W. and Kaback, H. R. and Knaff, D. and Krulwich, T. and Lodish, H. and Malmström, B. and Maloney, P. and Mannella, C. and Padan, E. and Papa, S. and Rottenberg, H. and Rudnick, G. and Rydström, J. and Silverstein, T. and Skulachev, V. and Slayman, C. L. and Tedeschi, H. and Wikström, M. and Wilson, T. H. (1993):
Transmembrane measurements across bioenergetic membranes.
In: Biochimica et Biophysica Acta, 1183 (1), pp. 1-3. Elsevier, ISSN 1879-2650,
[Article]

Bertl, A. and Slayman, C. L. and Gradmann, D. (1993):
Gating and conductance in an outward-rectifying K+ channel from the plasma membrane of Saccharomyces cerevisiae.
In: The Journal of membrane biology, 132 (3), pp. 183-199. ISSN 0022-2631,
[Article]

1992

Bertl, A. and Blumwald, E. and Coronado, R. and Eisenberg, R. and Findlay, G. and Gradmann, D. and Hille, B. and Köhler, K. and Kolb, H. A. and MacRobbie, E. (1992):
Electrical measurements on endomembranes.
In: Science (New York, N.Y.), 258 (5084), pp. 873-4. ISSN 0036-8075,
[Article]

Bertl, A. and Gradmann, D. and Slayman, C. L. (1992):
Calcium- and voltage-dependent ion channels in Saccharomyces cerevisiae.
In: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 338 (1283), pp. 63-72. ISSN 0962-8436,
[Article]

Bertl, A. and Slayman, C. L. (1992):
Complex modulation of cation channels in the tonoplast and plasma membrane of Saccharomyces cerevisiae: single-channel studies.
In: The Journal of experimental biology, 172, pp. 271-287. ISSN 0022-0949,
[Article]

Spalding, E. P. and Slayman, C. L. and Goldsmith, M. H. M. and Gradmann, D. and Bertl, A. (1992):
Ion channels in Arabidopsis plasma membrane : transport characteristics and involvement in light-induced voltage changes.
In: Plant physiology, 99 (1), pp. 96-102. ISSN 0032-0889,
[Article]

1990

Bertl, A. and Gradmann, D. Beilby, M. J. and Walker, N. A. and Smith, J. R. (eds.) International Workshop on Plant Membrane Transport (7th :, 1986 : Sydney, N.S.W.) (Corporate Creator) (1990):
Single-channel recordings from the plasmalemma of Acetabularia.
In: Membrane transport in plants and fungi : proceedings of 7th international workshop on plant membrane transport, Sydney, 1986, pp. 73-76, Sydney, University of Sydney, ISBN 0867584920,
[Book Section]

Bertl, A. and Slayman, C. L. (1990):
Cation-selective channels in the vacuolar membrane of Saccharomyces: dependence on calcium, redox state, and voltage.
In: Proceedings of the National Academy of Sciences of the United States of America, 87 (20), pp. 7824-7828. ISSN 0027-8424,
[Article]

1989

Bertl, A. (1989):
Current-voltage relationships of a sodium-sensitive potassium channel in the tonoplast of Chara corallina.
In: Journal of Membrane Biology, 109 (1), pp. 9-19. Springer, ISSN 0022-2631,
[Article]

Gradmann, D. and Bertl, A. (1989):
Physiological control of membrane currents in plants.
In: Plant Physiology and Biochemistry, 27, pp. 587-593. Elsevier, ISSN 0981-9428,
[Article]

1988

Bertl, A. and Klieber, H. G. and Gradmann, D. (1988):
Slow kinetics of a potassium channel in Acetabularia.
In: Journal of Membrane Biology, 102 (2), pp. 141-152. Springer, ISSN 0022-2631,
[Article]

1987

Bertl, A. and Gradmann, D. (1987):
Current-voltage relationships of potassium channels in the plasmalemma of Acetabularia.
In: Journal of Membrane Biology, 99 (1), pp. 41-49. Springer, ISSN 0022-2631,
[Article]

1986

Felle, H. and Bertl, A. (1986):
The fabrication of H+-selective liquid-membrane micro-electrodes for use in plant cells.
In: Journal of experimental botany, 37 (9), pp. 1416-1428. Oxford University Press, ISSN 1460-2431,
[Article]

Felle, H. and Bertl, A. (1986):
Light-induced cytoplasmic pH changes and their interrelation to the activity of the electrogenic proton pump in Riccia fluitans.
In: Biochimica et Biophysica Acta (BBA) - Bioenergetics, 848 (2), pp. 176-182. Elsevier, ISSN 1879-2650,
[Article]

Felle, H. and Brummer, B. and Bertl, A. and Parish, R. W. W. (1986):
Indole-3-acetic acid and fusicoccin cause cytosolic acidification of corn coleoptile cells.
In: Proceedings of the National Academy of Sciences of the United States of America, 83 (23), pp. 8992-8995. ISSN 0027-8424,
[Article]

1985

Brummer, B. and Bertl, A. and Potrykus, I. and Felle, H. and Parish, R. W. W. (1985):
Evidence that fusicoccin and indole-3-acetic acid induce cytosolic acidification of Zea mays cells.
In: FEBS Letters, 189 (1), pp. 109-114. Elsevier, ISSN 1873-3468,
[Article]

Felle, H. and Bertl, A. (1985):
Cytoplasmic pH of Root Hair Cells of Sinapis alba Recorded by a pH-sensitive Micro-electrode. Does Fusicoccin Stimulate the Proton Pump by Cytoplasmic Acidification?
In: Journal of experimental botany, 36 (7), pp. 1142-1149. Oxford University Press, ISSN 1460-2431,
[Article]

1984

Bertl, A. and Felle, H. and Bentrup, F. W. (1984):
Amine Transport in Riccia fluitans: Cytoplasmic and Vacuolar pH Recorded by a pH-Sensitive Microelectrode.
In: Plant physiology, 76 (1), pp. 75-78. ISSN 0032-0889,
[Article]

This list was generated on Wed Feb 24 01:50:05 2021 CET.